Plasmid

Part:BBa_K1312004:Design

Designed by: Yutaka Murata   Group: iGEM14_Kyoto   (2014-10-04)


pSB1C3 plus 6x His tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2070
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2070
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2076
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2070
    Illegal XhoI site found at 1054
    Illegal XhoI site found at 1946
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2070
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2070
    Illegal XbaI site found at 2085
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added 6xHistag and stop codon UAA to after Biobrick Suffix. When you use this part, you need to take care of cloning method. When you clone coding protein region, you have to clone this before stop codon. This is because if coding protein region has stop codon, ribosome stop transcripting at stop codon, so 6xHistag which is after Biobrick suffix isn't transcribed. If you clone protein coding gene just before stop codon, 6xHistag and stop codon which is after 6xHistag is transcribed in adequate reading frame.

Source

pSB1C3

References